To assess the hair growth in each group, photographs of the animals were taken at week 1, 2, 3, and 4 after topical application was begun. Both animal care and the protocol for this study were in accordance with IACUC (Institutional Animal Care and Use Committee) and OECD guidelines. Each compound (100 μl) was topically applied to the shaved dorsal area once a day, 6 days a week, for 4 weeks. All animals were randomized into 4 groups based on different topical applications: saline (SA), jojoba oil (JO), 3% minoxidil (MXD), and 3% peppermint oil (PEO, diluted in jojoba oil). Upon shaving the mice all of the hair follicles were synchronized in the telogen stage, showing pink color. The dorsal area (2 cm × 4 cm) of the 6-week-old C57BL/6 mice was shaved with an animal clipper. Five-week-old male C57BL/6 mice (Daehan Biolink Co., Korea) were allowed to adapt to their new environment for one week, with food and water provided ad libitum under 22 ± 1℃ room temperature, 50 ± 5% relative humidity and 12 hrs of a light/dark cycle before the experiment was begun. The aim of this study was to address the therapeutic potential of PEO for hair loss via the comparative analysis between PEO and minoxidil.Įxperimental animal. However, experimental trial of PEO in its hair growth activity has not been fully reported. Several clinical trials examining the effects of peppermint oil (PEO) on irritable bowel syndrome have been reported (9). In vitro, peppermint has been reported to show anti-inflammatory, antimicrobial, and antifungal activities as well as strong antioxidant activity, and antiallergenic and antitumor actions (7, 8). The principal ingredient of peppermint oil, menthol, is primarily responsible for its beneficial effects (6). Peppermint ( Mentha piperita) extracted from peppermint leaves is generally regarded as an excellent carminative and gastric stimulant, and also has been used in cosmetic formulations as a fragrance component and a general skin conditioning agent. Traditional plant remedies have been used for centuries in the treatment for hair loss, but only a few have been scientifically evaluated (5). Upjohn itself has warned of possible negative side effects of the medication including increased heart rate, difficulty breathing, rapid weight gain, edema, seborrhoeic dermatitis, scalp itching, and scaling (3- 5). Minoxidil was originally created as a hypertension medication by Upjohn Pharmaceuticals (2). The only products sanctioned by the US FDA for hair loss treatment are oral finasteride (Proscar ®) and topical minoxidil (Rogaine ®). For example, androgenetic alopecia in men, or male pattern baldness, is increasingly recognized as a physically and psychologically serious medical condition that often requires a professional care by generalist clinicians (1). Hair loss is a distressing condition that is associated with a multitude of natural, medical, or nutritional conditions. These results suggest that PEO induces a rapid anagen stage and could be used for a practical agent for hair growth without change of body weight gain and food efficiency. Body weight gain and food efficiency were not significantly different between groups. ALP activity and IGF-1 expression also significantly increased in PEO group. Of the 4 experimental groups, PEO group showed the most prominent hair growth effects a significant increase in dermal thickness, follicle number, and follicle depth. The hair growth effects of the 4-week topical applications were evaluated in terms of hair growth, histological analysis, enzymatic activity of alkaline phosphatase (ALP), and gene expression of insulin-like growth factor-1 (IGF-1), known bio-markers for the enhanced hair growth. The animals were randomized into 4 groups based on different topical applications: saline (SA), jojoba oil (JO), 3% minoxidil (MXD), and 3% peppermint oil (PEO). This study investigated the effect of peppermint oil on hair growth in C57BL/6 mice. This plant also has been used in cosmetic formulations as a fragrance component and skin conditioning agent. Peppermint ( Mentha piperita) is a plant native to Europe and has been widely used as a carminative and gastric stimulant worldwide.

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